Single‐Molecule mRNA Detection in Live Yeast
نویسندگان
چکیده
منابع مشابه
mRNA detection in budding yeast with single fluorophores
Quantitative measurement of mRNA levels in single cells is necessary to understand phenotypic variability within an otherwise isogenic population of cells. Single-molecule mRNA Fluorescence In Situ Hybridization (FISH) has been established as the standard method for this purpose, but current protocols require a long region of mRNA to be targeted by multiple DNA probes. Here, we introduce a new ...
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ژورنال
عنوان ژورنال: Current Protocols in Molecular Biology
سال: 2016
ISSN: 1934-3639,1934-3647
DOI: 10.1002/0471142727.mb1424s113